L-012化學(xué)發(fā)光探針
生化學(xué)用 for Biochemistry3116094040028274.png 規(guī)格含量 : 90.0+%（HPLC） 制造商 : FUJIFILM Wako Pure Chemical Corporation（富士膠片和光） 儲(chǔ)存條件：冷藏（干冰運(yùn)輸） CAS RN? : 143556-24-5 分子式 : C13H8ClN4NaO2 分子量 : 310.67 |  |
◆基本信息
L-012 是一種高靈敏度的化學(xué)發(fā)光（CHL）探針，比魯米諾（Luminol）試劑更有效。L-012 與多種類(lèi)型的活性氧反應(yīng)，這些活性氧來(lái)自人血液和口腔中激活的中性粒細(xì)胞或者大鼠腹膜腔。該產(chǎn)品可以用于有辣根過(guò)氧化物酶的ELISA 試劑盒，可提高反應(yīng)靈敏度。
結(jié)構(gòu)式
溶解及保存：蒸餾水溶解成20 mM/L（6.23 mg/mL）的水溶液，再用50 mM/L的Tris鹽酸緩沖液（pH 7.5）稀釋 至最終濃度為500 μM/L（有細(xì)胞的反應(yīng)體系）。另外，在沒(méi)細(xì)胞參與的反應(yīng)體系中時(shí)，建議使用終濃度為5~50 μM/L。母液冷凍保存，使用時(shí)再稀釋。
◆物性信息
外觀 | 白色~黃色，結(jié)晶~粉末 |
溶解性 | 可溶于水。 |
| 產(chǎn)品編號(hào) | 產(chǎn)品名稱(chēng) | 產(chǎn)品規(guī)格 | 產(chǎn)品等級(jí) |
| 120-04891 | L-012 L-012化學(xué)發(fā)光探針 | 100 mg | － |
拜爾迪是FUJIFILM wako授權(quán)代理商，
更多產(chǎn)品請(qǐng)咨詢(xún)：
北京拜爾迪生物技術(shù)有限公司
咨詢(xún)熱線：010-62960866
Q&A
Q： L-012能滲入細(xì)胞嗎?
A： 由于L-012對(duì)脂質(zhì)高度親和，它比luminol和lucigenin更能具有細(xì)胞滲透性。另一方面，ROS無(wú)限制地在胞外釋放。因此L-012被認(rèn)為也能
檢測(cè)胞外ROS。
Q： L-012的使用濃度如何?
A： 這取決于應(yīng)用和條件。
[體外] 培養(yǎng)細(xì)胞（EoL-1細(xì)胞）： 500 ~ 800 μmol/L.
全血和分離的中性粒細(xì)胞： 400 μmol/L
多形核中性粒細(xì)胞（PMNs）（由小鼠中分離）： 2 mmol/L
無(wú)細(xì)胞的樣品： 4 μmol/L, 15 μmol/L, and 50 μmol/L
[體內(nèi)] Balb/c 小鼠[皮下]： 20 ~ 80 μL of L-012(5mg/mL)
Balb/c 小鼠[腹膜內(nèi)]： 100 μL of L-012 (15mg/mL)
C57BL/10.Q (B10.Q) 小鼠 [腹膜內(nèi)]： 1 ~ 75 mg/kg
Q： 是否可用于動(dòng)物活體成像？
A： 如果所用的小動(dòng)物成像儀中有發(fā)光檢測(cè)探頭，就可以進(jìn)行檢測(cè)。以下是文獻(xiàn)報(bào)道過(guò)的儀器型號(hào)：
?IVIS 100 imaging systems
?Infinite M200 [Tecan]
?Luminescence Reader BLR-201 [Aloka]
?Beckman-Coulter DTX880 multimode plate reader [Beckman]
Q： 是否可用于固定細(xì)胞？
A： 目前尚無(wú)證據(jù)表明，活性氧（ROS）在細(xì)胞活性停止后仍然存在。普遍看法是認(rèn)為在細(xì)胞活性停止后（細(xì)胞死亡）ROS消失。固定組織或
細(xì)胞會(huì)停止細(xì)胞活性，因此不建議用于ROS檢測(cè)。
本品無(wú)需固定檢測(cè)對(duì)象即可檢測(cè)到ROS，使用方法包括但不限于將其添加到細(xì)胞培養(yǎng)上清液中或進(jìn)行動(dòng)物腹腔注射。
參考文獻(xiàn)
1. | Tsubasa Ogasahara, Yusuke Kouzai, Megumi Watanabe, Akihiro Takahashi, Kotaro Takahagi, June-Sik Kim, Hidenori Matsui, Mikihiro Yamamoto, Kazuhiro Toyoda, Yuki Ichinose, Keiichi Mochida, Yoshiteru Noutoshi Front Plant Sci. 2022; 13: 1004184. Published online 2022 Sep 15. doi: 10.3389/fpls.2022.1004184 PMCID: PMC9521188 |
2. | Hexin Wen, Xiaofeng Zhang, Qingqing Li, Ju Huang, Guangyong Liu, Jingyue Zhao, Yiran Liu, Li Shen, Yuyang Li, Kun Yang, Lugen Zuo, Jing Li, Jing Nian, Ping Xiang, Hao Zhao, Liang Yu, Mulin Liu, Zhijun Geng, Xue Song Oxid Med Cell Longev. 2022; 2022: 4877275. Published online 2022 Mar 10. doi: 10.1155/2022/4877275 PMCID: PMC8930266 |
3. | Fernando Navarrete, Michelle Gallei, Aleksandra E. Kornienko, Indira Saado, Mamoona Khan, Khong-Sam Chia, Martin A. Darino, Janos Bindics, Armin Djamei Plant Commun. 2022 Mar 14; 3(2): 100269. Published online 2021 Dec 17. doi: 10.1016/j.xplc.2021.100269 PMCID: PMC9073326 |
4. | Noninvasive imaging of the lung NETosis by anti-Ly6G iron oxide nanoparticles Jianghong Zhong, Chanyu Zheng, Haiqiang Gao, Wei Tong, Hui Hui, Jie Tian Heliyon. 2022 Aug; 8(8): e10043. Published online 2022 Aug 3. doi: 10.1016/j.heliyon.2022.e10043 PMCID: PMC9382280 |
5. | Jonathan R. Erlich, Eunice E. To, Raymond Luong, Felicia Liong, Stella Liong, Osezua Oseghale, Mark A. Miles, Steven Bozinovski, Robert D. Brooks, Ross Vlahos, Stanley Chan, John J. O’Leary, Doug A. Brooks, Stavros Selemidis Antioxidants (Basel) 2022 Aug; 11(8): 1488. Published online 2022 Jul 29. doi: 10.3390/antiox11081488 PMCID: PMC9405479 |
6. | Jennifer Kimball, Yaya Cui, Dongqin Chen, Pat Brown, William L. Rooney, Gary Stacey, Peter J. Balint-Kurti Sci Rep. 2019; 9: 18285. Published online 2019 Dec 4. doi: 10.1038/s41598-019-54802-x PMCID: PMC6893015 |
7. | Jiyoung Park, Tae-Houn Kim, Yohei Takahashi, Rebecca Schwab, Keini Dressano, Aaron B Stephan, Paulo HO Ceciliato, Eduardo Ramirez, Vince Garin, Alisa Huffaker, Julian I Schroeder Plant J. Author manuscript; available in PMC 2020 May 1. Published in final edited form as: Plant J. 2019 May; 98(3): 492–510. Published online 2019 Mar 7. doi: 10.1111/tpj.14232 PMCID: PMC6488365 |
8. | Eunice E. To, Jonathan Erlich, Felicia Liong, Raymond Luong, Stella Liong, Steven Bozinovski, Huei Jiunn Seow, John J. O’Leary, Doug A. Brooks, Ross Vlahos, Stavros Selemidis Sci Rep. 2019; 9: 2366. Published online 2019 Feb 20. doi: 10.1038/s41598-019-38864-5 PMCID: PMC6382773 |
9. | Sina Barghahn, Gregory Arnal, Namrata Jain, Elena Petutschnig, Harry Brumer, Volker Lipka Front Plant Sci. 2021; 12: 682439. Published online 2021 Jun 17. doi: 10.3389/fpls.2021.682439 PMCID: PMC8247929 |
10. | The Pleiades are a cluster of fungal effectors that inhibit host defenses Fernando Navarrete, Nenad Grujic, Alexandra Stirnberg, Indira Saado, David Aleksza, Michelle Gallei, Hazem Adi, André Alcantara, Mamoona Khan, Janos Bindics, Marco Trujillo, Armin Djamei PLoS Pathog. 2021 Jun; 17(6): e1009641. Published online 2021 Jun 24. doi: 10.1371/journal.ppat.1009641 PMCID: PMC8224859 |
11. | Rozalynne Samira, Jennifer A. Kimball, Luis Fernando Samayoa, James B. Holland, Tiffany M. Jamann, Patrick J. Brown, Gary Stacey, Peter J. Balint-Kurti Sci Rep. 2020; 10: 20817. Published online 2020 Nov 30. doi: 10.1038/s41598-020-77684-w PMCID: PMC7704633 |
12. | Engineering Smut Resistance in Maize by Site-Directed Mutagenesis of LIPOXYGENASE 3 Krishna Mohan Pathi, Philipp Rink, Nagaveni Budhagatapalli, Ruben Betz, Indira Saado, Stefan Hiekel, Martin Becker, Armin Djamei, Jochen Kumlehn Front Plant Sci. 2020; 11: 543895. Published online 2020 Oct 21. doi: 10.3389/fpls.2020.543895 PMCID: PMC7609844 |
13. | Rapid repeatable in vivo detection of retinal reactive oxygen species Ning Fan, Sean M. Silverman, Yang Liu, Xizhen Wang, Byung-Jin Kim, Liping Tang, Abbot F. Clark, Xuyang Liu, Iok-Hou Pang Exp Eye Res. Author manuscript; available in PMC 2018 Aug 1. Published in final edited form as: Exp Eye Res. 2017 Aug; 161: 71–81. Published online 2017 Jun 8. doi: 10.1016/j.exer.2017.06.004 PMCID: PMC5554724 |
14. | A rapid bioluminescence assay for measuring myeloperoxidase activity in human plasma Reece J. Goiffon, Sara C. Martinez, David Piwnica-Worms Nat Commun. 2015 Feb 10; 6: 6271. Published online 2015 Feb 10. doi: 10.1038/ncomms7271 PMCID: PMC4347050 |
15. | CRISPR/Cas9-mediated knockout of p22phox leads to loss of Nox1 and Nox4, but not Nox5 activity Kim-Kristin Prior, Matthias S. Leisegang, Ivana Josipovic, Oliver L?we, Ajay M. Shah, Norbert Weissmann, Katrin Schr?der, Ralf P. Brandes Redox Biol. 2016 Oct; 9: 287–295. Published online 2016 Aug 24. doi: 10.1016/j.redox.2016.08.013 PMCID: PMC5021817 |
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